Sample requirements
Minimum input, concentration, RIN and purity for bulk RNA-seq, small RNA, single-cell and spatial workflows. Including what we accept from degraded and FFPE material, where a standard RIN threshold does not apply.
Transcriptomics platform
RNA can be submitted in RNase-free water, RNA stabilization reagent, or 10mM Tris pH 8.0. Total RNA should be DNA-free, with A260/A280 ≥ 1.8, A260/A230 ≥ 1.8 and RIN ≥ 6. Ship on dry ice. These requirements apply to our Transcriptomics platform (bulk RNA-seq and RT-qPCR validation).
| Service | Sample type | Recommended quantity | Minimum quantity | Minimum concentration |
|---|---|---|---|---|
| Whole Transcriptome Sequencing | Total RNA | ≥ 3 µg | 1 µg | 20 ng/µL |
| Low-Input RNA Sequencing | Total RNA | ≥ 20 ng | 20 pg | 1 pg/µL |
| mRNA Sequencing | Total RNA | ≥ 500 ng | 200 ng | 20 ng/µL |
| Cells | ≥ 1×10⁶ | — | — | |
| Tissue | ≥ 50 mg | 10 mg | — | |
| Total RNA / LncRNA Sequencing | Total RNA | ≥ 2 µg | 500 ng | 50 ng/µL |
| Cells | ≥ 2×10⁶ | — | — | |
| Tissue | ≥ 500 mg | 100 mg | — | |
| CircRNA Sequencing (linear RNA digestion) | Total RNA | ≥ 5 µg | 2 µg | 50 ng/µL |
| Cells | ≥ 2×10⁶ | — | — | |
| Tissue | ≥ 500 mg | 100 mg | — | |
| Metatranscriptome | Total RNA | ≥ 4 µg | 3 µg | 50 ng/µL |
| Cells | ≥ 5×10⁶ | — | — | |
| Environmental samples | ≥ 1.5 g | — | — | |
| Bacterial RNA Sequencing | Total RNA | ≥ 1 µg | — | — |
| Cells | ≥ 1×10⁷ | — | — | |
| TCR-seq | Total RNA | ≥ 100 ng | — | 10 ng/µL |
| Ribo-seq | Cells | ≥ 5×10⁶–10⁷ | — | — |
| Tissue | ≥ 400 mg | 200 mg | — | |
| Dual RNA-seq | Total RNA | ≥ 1 µg | 200 ng | 10 ng/µL |
| Cells | ≥ 5×10⁶ | — | — | |
| Tissue | ≥ 500 mg | 100 mg | — | |
| Exosomal RNA Sequencing | Total RNA | ≥ 100 ng | — | 20 ng/µL |
| Cell supernatants | ≥ 15 mL | — | — | |
| Serum, plasma | ≥ 1 mL | — | — |
Exosomal RNA Sequencing above covers cargo analysis on our Exosomes & EVs platform. EV isolation and characterization (NTA, Western blot) use the same starting biofluid volumes. Confirm exact volume with us if you need isolation only, without RNA-seq.
Transcriptomics & Genomics platforms
ScRNA-seq and 10X Visium run on our Transcriptomics platform; scATAC-seq and single-cell genome sequencing run on our Genomics platform.
| Service | Sample type | Recommended quantity & quality | Minimum quantity & quality |
|---|---|---|---|
| ScRNA-seq | Single cell suspension, fresh tissue | 2×10⁶ cells | 1×10⁶ cells |
| SnRNA-seq | Snap-frozen tissue | ≥ 100 mg (contact us for details) | |
| 10X Visium Spatial Transcriptome | OCT-embedded tissue, FFPE | 6.5 mm × 6.5 mm section | |
| scATAC-seq | Single-cell nuclei suspension | 1×10⁶ · nuclei <40 µm, intact membrane, >95% intact proportion | 5×10⁵ |
| Single-cell suspension | ≥1×10⁶ · viability >80%, 700–1200 cells/µL | 5×10⁵ | |
| Fresh tissue | 200 mg | — | |
| Whole blood (EDTA) | ≥ 5 mL | — | |
| Single Cell Genome Sequencing | Cells | 1–10³ · stored in 1× PBS (no Ca²⁺/Mg²⁺), ≤2 µL volume | — |
| DNA | — | ≥ 0.5 pg | |
| ScRRBS | Cell lines, primary cells, fresh tissue, frozen cells | 200 µL PCR tubes, 5 µL lysate/tube, ≤1 µL buffer when collecting cells · ≥3 biological replicates | |
Not sure which service fits?