Sample requirements
Minimum input, concentration, RIN and purity for bulk RNA-seq, low-input workflows and exosomal RNA sequencing. Including what we accept from degraded and FFPE material, where a standard RIN threshold does not apply.
NGS & Molecular Biology platform
RNA can be submitted in RNase-free water, RNA stabilization reagent, or 10mM Tris pH 8.0. Total RNA should be DNA-free, with A260/A280 ≥ 1.8, A260/A230 ≥ 1.8 and RIN ≥ 6. Ship on dry ice. These requirements apply to our NGS & Molecular Biology platform (bulk RNA-seq and RT-qPCR validation).
| Service | Sample type | Recommended quantity | Minimum quantity | Minimum concentration |
|---|---|---|---|---|
| Whole Transcriptome / LncRNA Sequencing | Total RNA | ≥ 3 µg | 1 µg | 20 ng/µL |
| Low-Input RNA Sequencing | Total RNA | ≥ 20 ng | 1 ng | 50 pg/µL |
| mRNA Sequencing | Total RNA | ≥ 500 ng | 200 ng | 20 ng/µL |
| Cells | ≥ 1×10⁶ | — | — | |
| Tissue | ≥ 50 mg | 10 mg | — | |
| Metatranscriptome | Total RNA | ≥ 4 µg | 3 µg | 50 ng/µL |
| Cells | ≥ 5×10⁶ | — | — | |
| Environmental samples | ≥ 1.5 g | — | — | |
| Exosomal RNA Sequencing | Total RNA | ≥ 100 ng | — | 20 ng/µL |
| Cell supernatants | ≥ 15 mL | — | — | |
| Serum, plasma | ≥ 1 mL | — | — |
Exosomal RNA Sequencing above covers cargo analysis on our Exosomes & EVs platform. EV isolation and characterization (NTA, Western blot) use the same starting biofluid volumes. Confirm exact volume with us if you need isolation only, without RNA-seq.
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