Sample requirements
Minimum input, concentration, purity and integrity for whole-genome and exome sequencing, targeted panels and HLA typing. Every value is given here in full, not locked in a PDF.
Genomics platform
DNA can be submitted in DNase-free water, elution buffer, or 10mM Tris pH 8.0. Samples should be RNase-treated, show no degradation or contamination, and have an OD260/280 as close to 1.8–2.0 as possible. Ship with ice packs. These requirements apply to our Genomics platform.
| Service | Sample type | Recommended quantity | Minimum quantity | Minimum concentration |
|---|---|---|---|---|
| Whole Genome Sequencing | Genomic DNA | ≥ 500 ng | 200 ng | 10 ng/µL |
| Whole Genome Sequencing (PCR-free) | Genomic DNA | ≥ 1 µg | 500 ng | 20 ng/µL |
| Whole Exome Sequencing | Genomic DNA | ≥ 500 ng | 100 ng | 10 ng/µL |
| 16S / 18S / ITS Sequencing | Genomic DNA | ≥ 100 ng | 10 ng | 1 ng/µL |
| HLA Typing | Genomic DNA | ≥ 200 ng | — | — |
| Cells, PBMC | ≥ 1×10⁶ | — | — | |
| Blood | ≥ 1 mL | — | — | |
| Buccal swab | 2 swabs | — | — | |
| Dried blood spot (DBS) | 2 completely filled spots, 10 mm diameter each | — | — |
The 16S/18S/ITS Sequencing row above is delivered through our Metagenomics & Microbiome platform.
Not sure which service fits?